She is a qualified medical doctor, holding a Bachelor of Medicine and Bachelor of Surgery from the University of KwaZulu-Natal and a Master of Public Health in Maternal Child Health and Aging from the Harvard School of Public Health. She has also completed a Social Entrepreneurship Certificate Programme at the Gordon Institute of Business Science (GIBS).

Dr Motsepe has worked in private practice and in public sector hospitals. She also worked with the Wits Reproductive Health and HIV Institute which is a leading African research institute focusing on sexual, reproductive health and HIV research. She has also served as Chairperson of the Gauteng Health Department’s Accreditation Committee.

Dr Motsepe was appointed to the Board of Trustees of Cyril Ramaphosa Foundation in 2020 and serves as the Chairperson. She also serves as the Patron of the Early Care Foundation (previously ASHA Trust), a non-profit organisation which provides early childhood development support programmes for home-based crèches in disadvantaged communities. She also serves as patron of the South African Civil Society for Women’s Adolescents’ and Children’s Health (SACSoWACH), the Students Sponsorship Programme (SSP) and a trustee of the Hospice Association of the Witwatersrand. Dr Motsepe is a former member of the National Medical and Dental Association (NAMDA), and the boards of the Vaal Reefs Disaster Trust and the Kids Haven Foundation.